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Functionalized Polystyrene Nanomaterials for Biomedicinal Applications
Dolanský, Jiří ; Mosinger, Jiří (advisor) ; Dědic, Roman (referee) ; Zimčík, Petr (referee)
Nowadays, there is an increasing risk of bacterial infections from bacteria strains resistant towards antibiotics. Thus, it is of utmost importance to research novel therapies which can overcome this difficulty. The presented thesis focuses on the preparation, characterization and antibacterial evaluation of polystyrene polymer nanomaterials (nanofiber membranes and nanoparticles) modified with compounds that can efficiently inhibit bacterial growth either by their nature (polyethyleneimine) or by photoactivation upon visible light excitation (NO- photodonors, photosensitizers) and consequent production of highly reactive inorganic bactericidal species, nitric oxide (NO) and singlet oxygen (O2(1 g)). All materials were fully characterized by several independent methods. The concentrations of NO and O2(1 g) were measured by amperometric and time-resolved spectroscopic techniques and by variety of chemical analytic procedures. Due to the presence of bactericidal species and the efficient photogeneration of NO and O2(1 g) at physiological conditions, all materials exhibit strong antibacterial action tested on a Gram-negative bacterial strain Escherichia coli. Hence, these functionalized polymer nanomaterials may be intriguing systems for medical-, biological-, or environmental- application where a...
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